Single-Cell Transcriptomic Atlas of Primate Ovarian Aging.
Cell
; 180(3): 585-600.e19, 2020 02 06.
Article
en En
| MEDLINE
| ID: mdl-32004457
ABSTRACT
Molecular mechanisms of ovarian aging and female age-related fertility decline remain unclear. We surveyed the single-cell transcriptomic landscape of ovaries from young and aged non-human primates (NHPs) and identified seven ovarian cell types with distinct gene-expression signatures, including oocyte and six types of ovarian somatic cells. In-depth dissection of gene-expression dynamics of oocytes revealed four subtypes at sequential and stepwise developmental stages. Further analysis of cell-type-specific aging-associated transcriptional changes uncovered the disturbance of antioxidant signaling specific to early-stage oocytes and granulosa cells, indicative of oxidative damage as a crucial factor in ovarian functional decline with age. Additionally, inactivated antioxidative pathways, increased reactive oxygen species, and apoptosis were observed in granulosa cells from aged women. This study provides a comprehensive understanding of the cell-type-specific mechanisms underlying primate ovarian aging at single-cell resolution, revealing new diagnostic biomarkers and potential therapeutic targets for age-related human ovarian disorders.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ovario
/
Envejecimiento
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Análisis de la Célula Individual
/
Transcriptoma
Tipo de estudio:
Prognostic_studies
/
Qualitative_research
Límite:
Aged
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Animals
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Female
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Humans
Idioma:
En
Revista:
Cell
Año:
2020
Tipo del documento:
Article