Restricted access carbon nanotube for microextraction by packed sorbent to determine antipsychotics in plasma samples by high-performance liquid chromatography-tandem mass spectrometry.
Anal Bioanal Chem
; 412(11): 2465-2475, 2020 Apr.
Article
en En
| MEDLINE
| ID: mdl-32025768
ABSTRACT
This manuscript describes the development of the restricted access carbon nanotube (RACNT) as a selective stationary phase for microextraction by packed sorbent (MEPS) to determine antipsychotics (chlorpromazine, clozapine, olanzapine, and quetiapine) in untreated plasma samples from schizophrenic patients by ultra-high liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The synthesis was achieved by chemically covering commercial multi-walled carbon nanotubes with bovine serum albumin (BSA) to subsequently pack the material in a polyethylene conical tube (1000 µL). The RACNTs' sorbents were able to exclude about 97% of the plasma proteins, maintaining the same performance for about 100 assays. The MEPS variables (sample pH, draw-eject cycles, desorption and phase cleanup) were evaluated to improve sensibility and selectivity. The MEPS/UHPLC-MS/MS method was linear at concentrations ranging from the lower limit of quantification (10.0 ng mL-1) to the upper limit of quantification (200-700 ng mL-1) with coefficients of determinations higher than 0.99. The precision assays presented relative standard deviation (RSD) values lower than 13%, and the accuracy assays presented relative error (RE) values that ranged from - 8.01 to 11.53%. Neither significant matrix effects nor carryover was observed. The developed method was successfully applied to determine antipsychotics drugs for therapeutic drug monitoring of schizophrenic patients.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Antipsicóticos
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Monitoreo de Drogas
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Nanotubos de Carbono
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Espectrometría de Masas en Tándem
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Microextracción en Fase Sólida
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2020
Tipo del documento:
Article
País de afiliación:
Brasil