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Ultrasensitive Genetically Encoded Indicator for Hydrogen Peroxide Identifies Roles for the Oxidant in Cell Migration and Mitochondrial Function.
Pak, Valeriy V; Ezerina, Daria; Lyublinskaya, Olga G; Pedre, Brandán; Tyurin-Kuzmin, Pyotr A; Mishina, Natalie M; Thauvin, Marion; Young, David; Wahni, Khadija; Martínez Gache, Santiago Agustín; Demidovich, Alexandra D; Ermakova, Yulia G; Maslova, Yulia D; Shokhina, Arina G; Eroglu, Emrah; Bilan, Dmitry S; Bogeski, Ivan; Michel, Thomas; Vriz, Sophie; Messens, Joris; Belousov, Vsevolod V.
Afiliación
  • Pak VV; Department of Metabolism and Redox Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia; Institute for Cardiovascular Physiology, Georg August University Göttingen, Göttingen 37073, Germany.
  • Ezerina D; VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnologie - Vrije Universiteit Brussel, B-1050 Brussels, Belgium; Brussels Center for Redox Biology, Vrije Universiteit Brussel, B-1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, B-1050 Brussels, Belgi
  • Lyublinskaya OG; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, St. Petersburg 194064, Russia.
  • Pedre B; VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnologie - Vrije Universiteit Brussel, B-1050 Brussels, Belgium; Brussels Center for Redox Biology, Vrije Universiteit Brussel, B-1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, B-1050 Brussels, Belgi
  • Tyurin-Kuzmin PA; Faculty of Medicine, Lomonosov Moscow State University, Moscow 119991, Russia.
  • Mishina NM; Department of Metabolism and Redox Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia.
  • Thauvin M; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, Paris 75231, France; Sorbonne Université, Collège Doctoral, Paris 75005, France.
  • Young D; VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnologie - Vrije Universiteit Brussel, B-1050 Brussels, Belgium; Brussels Center for Redox Biology, Vrije Universiteit Brussel, B-1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, B-1050 Brussels, Belgi
  • Wahni K; VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnologie - Vrije Universiteit Brussel, B-1050 Brussels, Belgium; Brussels Center for Redox Biology, Vrije Universiteit Brussel, B-1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, B-1050 Brussels, Belgi
  • Martínez Gache SA; VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnologie - Vrije Universiteit Brussel, B-1050 Brussels, Belgium; Brussels Center for Redox Biology, Vrije Universiteit Brussel, B-1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, B-1050 Brussels, Belgi
  • Demidovich AD; Department of Metabolism and Redox Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia.
  • Ermakova YG; Department of Metabolism and Redox Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia; European Molecular Biology Laboratory, Heidelberg 69117, Germany.
  • Maslova YD; Department of Metabolism and Redox Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia.
  • Shokhina AG; Department of Metabolism and Redox Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia.
  • Eroglu E; Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Bilan DS; Department of Metabolism and Redox Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Moscow 117997, Russia.
  • Bogeski I; Institute for Cardiovascular Physiology, Georg August University Göttingen, Göttingen 37073, Germany.
  • Michel T; Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Vriz S; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, Paris 75231, France; University Paris-Diderot, Paris 75006, France.
  • Messens J; VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnologie - Vrije Universiteit Brussel, B-1050 Brussels, Belgium; Brussels Center for Redox Biology, Vrije Universiteit Brussel, B-1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, B-1050 Brussels, Belgi
  • Belousov VV; Department of Metabolism and Redox Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Moscow 117997, Russia; Institute for Cardiov
Cell Metab ; 31(3): 642-653.e6, 2020 03 03.
Article en En | MEDLINE | ID: mdl-32130885
Hydrogen peroxide (H2O2) is a key redox intermediate generated within cells. Existing probes for H2O2 have not solved the problem of detection of the ultra-low concentrations of the oxidant: these reporters are not sensitive enough, or pH-dependent, or insufficiently bright, or not functional in mammalian cells, or have poor dynamic range. Here we present HyPer7, the first bright, pH-stable, ultrafast, and ultrasensitive ratiometric H2O2 probe. HyPer7 is fully functional in mammalian cells and in other higher eukaryotes. The probe consists of a circularly permuted GFP integrated into the ultrasensitive OxyR domain from Neisseria meningitidis. Using HyPer7, we were able to uncover the details of H2O2 diffusion from the mitochondrial matrix, to find a functional output of H2O2 gradients in polarized cells, and to prove the existence of H2O2 gradients in wounded tissue in vivo. Overall, HyPer7 is a probe of choice for real-time H2O2 imaging in various biological contexts.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Movimiento Celular / Oxidantes / Peróxido de Hidrógeno / Mitocondrias Límite: Animals / Humans Idioma: En Revista: Cell Metab Asunto de la revista: METABOLISMO Año: 2020 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Movimiento Celular / Oxidantes / Peróxido de Hidrógeno / Mitocondrias Límite: Animals / Humans Idioma: En Revista: Cell Metab Asunto de la revista: METABOLISMO Año: 2020 Tipo del documento: Article País de afiliación: Alemania