Epigenetic Regulation of WNT3A Enhancer during Regeneration of Injured Cortical Neurons.
Int J Mol Sci
; 21(5)2020 Mar 10.
Article
en En
| MEDLINE
| ID: mdl-32164275
ABSTRACT
Traumatic brain injury is known to reprogram the epigenome. Chromatin immunoprecipitation-sequencing of histone H3 lysine 27 acetylation (H3K27ac) and tri-methylation of histone H3 at lysine 4 (H3K4me3) marks was performed to address the transcriptional regulation of candidate regeneration-associated genes. In this study, we identify a novel enhancer region for induced WNT3A transcription during regeneration of injured cortical neurons. We further demonstrated an increased mono-methylation of histone H3 at lysine 4 (H3K4me1) modification at this enhancer concomitant with a topological interaction between sub-regions of this enhancer and with promoter of WNT3A gene. Together, this study reports a novel mechanism for WNT3A gene transcription and reveals a potential therapeutic intervention for neuronal regeneration.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Histonas
/
Proteína Wnt3A
/
Lesiones Traumáticas del Encéfalo
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
Int J Mol Sci
Año:
2020
Tipo del documento:
Article
País de afiliación:
Taiwán