High-resolution optoacoustic imaging of tissue responses to vascular-targeted therapies.
Nat Biomed Eng
; 4(3): 286-297, 2020 03.
Article
en En
| MEDLINE
| ID: mdl-32165736
ABSTRACT
The monitoring of vascular-targeted therapies using magnetic resonance imaging, computed tomography or ultrasound is limited by their insufficient spatial resolution. Here, by taking advantage of the intrinsic optical properties of haemoglobin, we show that raster-scanning optoacoustic mesoscopy (RSOM) provides high-resolution images of the tumour vasculature and of the surrounding tissue, and that the detection of a wide range of ultrasound bandwidths enables the distinction of vessels of differing size, providing detailed insights into the vascular responses to vascular-targeted therapy. Using RSOM to examine the responses to vascular-targeted photodynamic therapy in mice with subcutaneous xenografts, we observed a substantial and immediate occlusion of the tumour vessels followed by haemorrhage within the tissue and the eventual collapse of the entire vasculature. Using dual-wavelength RSOM, which distinguishes oxyhaemoglobin from deoxyhaemoglobin, we observed an increase in oxygenation of the entire tumour volume immediately after the application of the therapy, and a second wave of oxygen reperfusion approximately 24 h thereafter. We also show that RSOM enables the quantification of differences in neoangiogenesis that predict treatment efficacy.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Diagnóstico por Imagen
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Ultrasonografía
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Neoplasias Vasculares
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Técnicas Fotoacústicas
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Neovascularización Patológica
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Nat Biomed Eng
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos