Novel NanoLuc-type substrates with various C-6 substitutions.
Bioorg Med Chem Lett
; 30(9): 127085, 2020 05 01.
Article
en En
| MEDLINE
| ID: mdl-32171617
ABSTRACT
NanoLuc (NLuc)-furimazine bioluminescence system offers several advantages over established systems, including improved stability, smaller size, and >150-fold enhancement in bioluminescence. Herein, we designed and synthesized a series of bioluminescent substrates with varying at the C-6 position of furimazine for NLuc-furimazine bioluminescence system. Among all derivatives, compounds A6 and A11 provided excellent bioluminescence characteristics compared with furimazine in vitro and in vivo. We believe that these new NLuc substrates can broaden the application of NLuc bioluminescence techniques, especially in vivo bioluminescent imaging.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Pirazinas
/
Diseño de Fármacos
/
Furanos
/
Imidazoles
/
Luciferasas
/
Mediciones Luminiscentes
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Bioorg Med Chem Lett
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
China