Current Understanding of the Mechanism of Water Oxidation in Photosystem II and Its Relation to XFEL Data.
Annu Rev Biochem
; 89: 795-820, 2020 06 20.
Article
en En
| MEDLINE
| ID: mdl-32208765
ABSTRACT
The investigation of water oxidation in photosynthesis has remained a central topic in biochemical research for the last few decades due to the importance of this catalytic process for technological applications. Significant progress has been made following the 2011 report of a high-resolution X-ray crystallographic structure resolving the site of catalysis, a protein-bound Mn4CaOx complex, which passes through ≥5 intermediate states in the water-splitting cycle. Spectroscopic techniques complemented by quantum chemical calculations aided in understanding the electronic structure of the cofactor in all (detectable) states of the enzymatic process. Together with isotope labeling, these techniques also revealed the binding of the two substrate water molecules to the cluster. These results are described in the context of recent progress using X-ray crystallography with free-electron lasers on these intermediates. The data are instrumental for developing a model for the biological water oxidation cycle.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Oxígeno
/
Agua
/
Coenzimas
/
Complejo de Proteína del Fotosistema II
/
Manganeso
Idioma:
En
Revista:
Annu Rev Biochem
Año:
2020
Tipo del documento:
Article