In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system.

Chen, Yili; Jiang, Hongye; Wang, Ting; He, Dan; Tian, Rui; Cui, Zifeng; Tian, Xun; Gao, Qinglei; Ma, Xin; Yang, Jianrong; Wu, Jun; Tan, Songwei; Xu, Hongyan; Tang, Xiongzhi; Wang, Yan; Yu, Zhiying; Han, Hui; Das, Bhudev C; Severinov, Konstantin; Hitzeroth, Inga Isabel; Debata, Priya Ranjan; Xu, Wei; Fan, Weiwen; Jin, Zhuang; Cao, Chen; Yu, Miao; Xie, Weiling; Huang, Zhaoyue; Hu, Zheng; You, Zeshan

Chen, Yili; Jiang, Hongye; Wang, Ting; He, Dan; Tian, Rui; Cui, Zifeng; Tian, Xun; Gao, Qinglei; Ma, Xin; Yang, Jianrong; Wu, Jun; Tan, Songwei; Xu, Hongyan; Tang, Xiongzhi; Wang, Yan; Yu, Zhiying; Han, Hui; Das, Bhudev C; Severinov, Konstantin; Hitzeroth, Inga Isabel; Debata, Priya Ranjan; Xu, Wei; Fan, Weiwen; Jin, Zhuang; Cao, Chen; Yu, Miao; Xie, Weiling; Huang, Zhaoyue; Hu, Zheng; You, Zeshan.

Afiliación

Chen Y; Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Jiang H; Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Wang T; Department of Anesthesia Operation, Zhongnan Hospital of Wuhan University, Wuhan, China.
He D; Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Tian R; Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Cui Z; Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Tian X; Department of Obstetrics and Gynecology, Academician Expert Workstation, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Gao Q; Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.
Ma X; Department of Urology, The General Hospital of the People's Liberation Army, Beijing, China.
Yang J; Department of Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
Wu J; School of Biomedical Engineering, Sun Yat-sen University, Guangzhou, China.
Tan S; Tongji School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Xu H; Department of Obstetrics and Gynecology, Yuebei People's Hospital, Medical College of Shantou University, Shaoguan, Guangdong, China.
Tang X; Department of Gynecology, Guilin People's Hospital, Guilin, The Guangxi Zhuang Autonomous Region, China.
Wang Y; Key Laboratory of Molecular Biophysics of the Ministry of Education, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Yu Z; Department of Obstetrics & Gynecology, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, China.
Han H; State Key Laboratory of Oncology in Southern China, Collaborative Innovation Center for Cancer Medicine & Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.
Das BC; Amity Institute of Molecular Medicine & Stem Cell Research, Amity University, Uttar Pradesh, Noida, 201313, India.
Severinov K; Skolkovo Institute of Science and Technology, Skolkovo, Moscow Region, 143025, Russia.
Hitzeroth II; Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Cape Town, 7701, South Africa.
Debata PR; Department of Zoology, North Orissa University, Takatpur, Baripada, Odisha, 757003, India.
Xu W; Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Fan W; Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Jin Z; Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Cao C; Department of Obstetrics and Gynecology, Academician Expert Workstation, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Yu M; Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Xie W; Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Huang Z; Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Hu Z; Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China; Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China. Electronic addre
You Z; Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China. Electronic address: youzeshan888@hotmail.com.

Antiviral Res ; 178: 104794, 2020 06.

Article en En | MEDLINE | ID: mdl-32298665

Asunto(s)

Sistemas CRISPR-Cas; Proteínas de Unión al ADN/genética; Proteínas Oncogénicas Virales/genética; Proteínas E7 de Papillomavirus/genética; ARN Mensajero/metabolismo; Proteínas Represoras/genética; Neoplasias del Cuello Uterino/patología; Animales; Apoptosis; Línea Celular Tumoral; Proliferación Celular; Roturas del ADN de Doble Cadena; Regulación hacia Abajo; Femenino; Terapia Genética; Células HeLa; Papillomavirus Humano 16/genética; Papillomavirus Humano 18/genética; Humanos; Ratones; Ratones Endogámicos BALB C; Infecciones por Papillomavirus/virología; ARN Mensajero/genética; ARN Viral/genética; ARN Viral/metabolismo; Proteínas de Unión a Retinoblastoma/genética; Proteína p53 Supresora de Tumor/genética; Ubiquitina-Proteína Ligasas/genética; Regulación hacia Arriba; Neoplasias del Cuello Uterino/virología; Ensayos Antitumor por Modelo de Xenoinjerto

Palabras clave

CRISPR/Cas13a system; Cervical cancer; E6/E7; Growth inhibition; HPV 16/18; Tumor suppression

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteínas Represoras / ARN Mensajero / Neoplasias del Cuello Uterino / Proteínas Oncogénicas Virales / Proteínas de Unión al ADN / Proteínas E7 de Papillomavirus / Sistemas CRISPR-Cas Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Antiviral Res Año: 2020 Tipo del documento: Article País de afiliación: China

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