Toward a Universal Sample Preparation Method for Denaturing Top-Down Proteomics of Complex Proteomes.

Yang, Zhichang; Shen, Xiaojing; Chen, Daoyang; Sun, Liangliang

Yang, Zhichang; Shen, Xiaojing; Chen, Daoyang; Sun, Liangliang.

Afiliación

Yang Z; Department of Chemistry, Michigan State University, 578 S Shaw Ln, East Lansing, Michigan 48824 United States.
Shen X; Department of Chemistry, Michigan State University, 578 S Shaw Ln, East Lansing, Michigan 48824 United States.
Chen D; Department of Chemistry, Michigan State University, 578 S Shaw Ln, East Lansing, Michigan 48824 United States.
Sun L; Department of Chemistry, Michigan State University, 578 S Shaw Ln, East Lansing, Michigan 48824 United States.

J Proteome Res ; 19(8): 3315-3325, 2020 08 07.

Article en En | MEDLINE | ID: mdl-32419461

Asunto(s)

Proteínas de Escherichia coli; Proteoma; Escherichia coli/genética; Humanos; Proteómica; Reproducibilidad de los Resultados; Espectrometría de Masas en Tándem

Palabras clave

CZE-MS/MS; PTMs; SDS; SP3; chloroform−methanol precipitation; denaturing top-down proteomics; histone; membrane proteins; membrane ultrafiltration; sample preparation

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteoma / Proteínas de Escherichia coli Límite: Humans Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2020 Tipo del documento: Article

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