Integrating whole-genome sequencing within the National Antimicrobial Resistance Surveillance Program in the Philippines.
Nat Commun
; 11(1): 2719, 2020 06 01.
Article
en En
| MEDLINE
| ID: mdl-32483195
ABSTRACT
National networks of laboratory-based surveillance of antimicrobial resistance (AMR) monitor resistance trends and disseminate these data to AMR stakeholders. Whole-genome sequencing (WGS) can support surveillance by pinpointing resistance mechanisms and uncovering transmission patterns. However, genomic surveillance is rare in low- and middle-income countries. Here, we implement WGS within the established Antimicrobial Resistance Surveillance Program of the Philippines via a binational collaboration. In parallel, we characterize bacterial populations of key bug-drug combinations via a retrospective sequencing survey. By linking the resistance phenotypes to genomic data, we reveal the interplay of genetic lineages (strains), AMR mechanisms, and AMR vehicles underlying the expansion of specific resistance phenotypes that coincide with the growing carbapenem resistance rates observed since 2010. Our results enhance our understanding of the drivers of carbapenem resistance in the Philippines, while also serving as the genetic background to contextualize ongoing local prospective surveillance.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Genoma Bacteriano
/
Genómica
/
Farmacorresistencia Bacteriana
/
Secuenciación Completa del Genoma
/
Antibacterianos
Tipo de estudio:
Screening_studies
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Reino Unido