Production of Active Recombinant Hyaluronidase Inclusion Bodies from <i>Apis mellifera</i> in <i>E. coli</i> Bl21(DE3) and characterization by FT-IR Spectroscopy.

Schwaighofer, Andreas; Ablasser, Sarah; Lux, Laurin; Kopp, Julian; Herwig, Christoph; Spadiut, Oliver; Lendl, Bernhard; Slouka, Christoph

Production of Active Recombinant Hyaluronidase Inclusion Bodies from Apis mellifera in E. coli Bl21(DE3) and characterization by FT-IR Spectroscopy.

Schwaighofer, Andreas; Ablasser, Sarah; Lux, Laurin; Kopp, Julian; Herwig, Christoph; Spadiut, Oliver; Lendl, Bernhard; Slouka, Christoph.

Afiliación

Schwaighofer A; FG Environmental Analytics, Process Analytics and Sensors, Institute of Chemical Technology and Analytics, Vienna University of Technology, Getreidemarkt 9/164, 1060 Wien, Austria.
Ablasser S; FG Bioprocess Technology, ICEBE, Vienna University of Technology, Gumpendorferstrasse 1a, 1060 Vienna, Austria.
Lux L; FG Environmental Analytics, Process Analytics and Sensors, Institute of Chemical Technology and Analytics, Vienna University of Technology, Getreidemarkt 9/164, 1060 Wien, Austria.
Kopp J; FG Bioprocess Technology, ICEBE, Vienna University of Technology, Gumpendorferstrasse 1a, 1060 Vienna, Austria.
Herwig C; FG Bioprocess Technology, ICEBE, Vienna University of Technology, Gumpendorferstrasse 1a, 1060 Vienna, Austria.
Spadiut O; FG Integrated Bioprocess Development, ICEBE, Vienna University of Technology, Gumpendorferstrasse 1a, 1060 Vienna, Austria.
Lendl B; FG Environmental Analytics, Process Analytics and Sensors, Institute of Chemical Technology and Analytics, Vienna University of Technology, Getreidemarkt 9/164, 1060 Wien, Austria.
Slouka C; FG Integrated Bioprocess Development, ICEBE, Vienna University of Technology, Gumpendorferstrasse 1a, 1060 Vienna, Austria.

Int J Mol Sci ; 21(11)2020 May 29.

Article en En | MEDLINE | ID: mdl-32485932

Asunto(s)

Escherichia coli/metabolismo; Hialuronoglucosaminidasa/biosíntesis; Cuerpos de Inclusión/metabolismo; Proteínas Recombinantes/biosíntesis; Animales; Abejas; Biomasa; Reactores Biológicos; Medios de Cultivo/metabolismo; Disulfuros; Fermentación; Ácido Hialurónico/metabolismo; Peso Molecular; Espectroscopía Infrarroja por Transformada de Fourier; Especificidad por Sustrato; Temperatura

Palabras clave

Escherichia coli; FT-IR spectroscopy; active inclusion body; recombinant protein production; upstream process development

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Cuerpos de Inclusión / Escherichia coli / Hialuronoglucosaminidasa Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: Austria

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