Coordination of transcriptional and translational regulations in human epithelial cells infected by Listeria monocytogenes.
RNA Biol
; 17(10): 1492-1507, 2020 10.
Article
en En
| MEDLINE
| ID: mdl-32584699
ABSTRACT
The invasion of mammalian cells by intracellular bacterial pathogens reshuffles their gene expression and functions; however, we lack dynamic insight into the distinct control levels that shape the host response. Here, we have addressed the respective contribution of transcriptional and translational regulations during a time-course of infection of human intestinal epithelial cells by an epidemic strain of Listeria monocytogenes, using transcriptome analysis paralleled with ribosome profiling. Upregulations were dominated by early transcriptional activation of pro-inflammatory genes, whereas translation inhibition appeared as the major driver of downregulations. Instead of a widespread but transient shutoff, translation inhibition affected specifically and durably transcripts encoding components of the translation machinery harbouring a 5'-terminal oligopyrimidine motif. Pre-silencing the most repressed target gene (PABPC1) slowed down the intracellular multiplication of Listeria monocytogenes, suggesting that the infected host cell can benefit from the repression of genes involved in protein synthesis and thereby better control infection.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Transcripción Genética
/
Biosíntesis de Proteínas
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Células Epiteliales
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Interacciones Huésped-Patógeno
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Listeria monocytogenes
Límite:
Humans
Idioma:
En
Revista:
RNA Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2020
Tipo del documento:
Article
País de afiliación:
Francia