Detection of Basophils and Other Granulocytes in Induced Sputum by Flow Cytometry.
Methods Mol Biol
; 2163: 281-291, 2020.
Article
en En
| MEDLINE
| ID: mdl-32766984
ABSTRACT
Flow cytometry is one of the most widely used techniques for the detection of surface markers on various cells, particularly the cells of the immune system, at a single-cell resolution. Modern flow cytometers can identify rare cell population in highly heterogeneous samples. Here we present a protocol that allows a precise detection of basophils as well as eosinophils and neutrophils in induced sputum samples. The identification of sputum basophils and other granulocytes contributes to a better understanding of the cellular network that promotes and regulates inflammation of the lower respiratory tract.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Esputo
/
Basófilos
/
Inmunofenotipificación
/
Citometría de Flujo
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2020
Tipo del documento:
Article
País de afiliación:
Suiza