Deletion of yeast TPK1 reduces the efficiency of non-homologous end joining DNA repair.
Biochem Biophys Res Commun
; 533(4): 899-904, 2020 12 17.
Article
en En
| MEDLINE
| ID: mdl-33008596
ABSTRACT
Non-homologous end joining (NHEJ) is a highly conserved mechanism of DNA double-stranded break (DSB) repair. Here we utilize a computational protein-protein interaction method to identify human PRKACB as a potential candidate interacting with NHEJ proteins. We show that the deletion of its yeast homolog, TPK1 that codes for the protein kinase A catalytic subunit reduces the efficiency of NHEJ repair of breaks with overhangs and blunt ends in plasmid-based repair assays. Additionally, tpk1Δ mutants showed defects in the repair of chromosomal breaks induced by HO-site specific endonuclease. Our double deletion mutant analyses suggest that TPK1 and YKU80, a key player in NHEJ could function in parallel pathways. Altogether, here we report a novel involvement for TPK1 in NHEJ.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Proteínas Quinasas Dependientes de AMP Cíclico
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Proteínas de Saccharomyces cerevisiae
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Reparación del ADN por Unión de Extremidades
Límite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2020
Tipo del documento:
Article
País de afiliación:
Canadá