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Decreased Histone Acetylation Levels at Th1 and Regulatory Loci after Induction of Food Allergy.
Alashkar Alhamwe, Bilal; Meulenbroek, Laura A P M; Veening-Griffioen, Désirée H; Wehkamp, Tjalling M D; Alhamdan, Fahd; Miethe, Sarah; Harb, Hani; Hogenkamp, Astrid; Knippels, Léon M J; Pogge von Strandmann, Elke; Renz, Harald; Garssen, Johan; van Esch, Betty C A M; Garn, Holger; Potaczek, Daniel P; Tiemessen, Machteld M.
Afiliación
  • Alashkar Alhamwe B; Institute of Laboratory Medicine, the German Center for Lung Research (DZL) and the Universities of Giessen and Marburg Lung Center (UGMLC), Philipps University Marburg, 35039 Marburg, Germany.
  • Meulenbroek LAPM; Institute of Tumor Immunology, Clinic for Hematology, Oncology and Immunology, Center for Tumor Biology and Immunology, Philipps University Marburg, 35039 Marburg, Germany.
  • Veening-Griffioen DH; College of Pharmacy, International University for Science and Technology (IUST), Daraa 15, Syria.
  • Wehkamp TMD; Danone Nutricia Research, 3584 CT Utrecht, The Netherlands.
  • Alhamdan F; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CT Utrecht, The Netherlands.
  • Miethe S; Danone Nutricia Research, 3584 CT Utrecht, The Netherlands.
  • Harb H; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CT Utrecht, The Netherlands.
  • Hogenkamp A; Danone Nutricia Research, 3584 CT Utrecht, The Netherlands.
  • Knippels LMJ; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CT Utrecht, The Netherlands.
  • Pogge von Strandmann E; Institute of Laboratory Medicine, the German Center for Lung Research (DZL) and the Universities of Giessen and Marburg Lung Center (UGMLC), Philipps University Marburg, 35039 Marburg, Germany.
  • Renz H; Translational Inflammation Research Division & Core Facility for Single Cell Multiomics, the German Center for Lung Research (DZL), Universities of Giessen and Marburg Lung Center, Philipps University Marburg, 35039 Marburg, Germany.
  • Garssen J; Institute of Laboratory Medicine, the German Center for Lung Research (DZL) and the Universities of Giessen and Marburg Lung Center (UGMLC), Philipps University Marburg, 35039 Marburg, Germany.
  • van Esch BCAM; Translational Inflammation Research Division & Core Facility for Single Cell Multiomics, the German Center for Lung Research (DZL), Universities of Giessen and Marburg Lung Center, Philipps University Marburg, 35039 Marburg, Germany.
  • Garn H; Institute of Laboratory Medicine, the German Center for Lung Research (DZL) and the Universities of Giessen and Marburg Lung Center (UGMLC), Philipps University Marburg, 35039 Marburg, Germany.
  • Potaczek DP; Division of Immunology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Tiemessen MM; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CT Utrecht, The Netherlands.
Nutrients ; 12(10)2020 Oct 19.
Article en En | MEDLINE | ID: mdl-33086571
ABSTRACT
Immunoglobulin E (IgE)-mediated allergy against cow's milk protein fractions such as whey is one of the most common food-related allergic disorders of early childhood. Histone acetylation is an important epigenetic mechanism, shown to be involved in the pathogenesis of allergies. However, its role in food allergy remains unknown. IgE-mediated cow's milk allergy was successfully induced in a mouse model, as demonstrated by acute allergic symptoms, whey-specific IgE in serum, and the activation of mast cells upon a challenge with whey protein. The elicited allergic response coincided with reduced percentages of regulatory T (Treg) and T helper 17 (Th17) cells, matching decreased levels of H3 and/or H4 histone acetylation at pivotal Treg and Th17 loci, an epigenetic status favoring lower gene expression. In addition, histone acetylation levels at the crucial T helper 1 (Th1) loci were decreased, most probably preceding the expected reduction in Th1 cells after inducing an allergic response. No changes were observed for T helper 2 cells. However, increased histone acetylation levels, promoting gene expression, were observed at the signal transducer and activator of transcription 6 (Stat6) gene, a proallergic B cell locus, which was in line with the presence of whey-specific IgE. In conclusion, the observed histone acetylation changes are pathobiologically in line with the successful induction of cow's milk allergy, to which they might have also contributed mechanistically.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Inmunoglobulina E / Histonas / Hipersensibilidad a la Leche / Células TH1 Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Nutrients Año: 2020 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Inmunoglobulina E / Histonas / Hipersensibilidad a la Leche / Células TH1 Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Nutrients Año: 2020 Tipo del documento: Article País de afiliación: Alemania