Desmin and CD31 immunolabeling for detecting venous invasion of the pancreatobiliary tract cancers.
PLoS One
; 15(11): e0242571, 2020.
Article
en En
| MEDLINE
| ID: mdl-33253282
ABSTRACT
Although venous invasion (VI) is a poor prognostic factor for patients with pancreatobiliary tract cancers, its histopathologic characteristics have not been well described. We evaluated the patterns of VI and the added benefit provided by CD31, desmin, and dual CD31âdesmin immunolabeling for identification of VI. We included 120 surgically resected pancreatobiliary tract cancer cases-59 cases as a test set with known VI and 61 cases as a validation set without information of VI. VI was classified into three patterns intraepithelial neoplasia-like (IN-like), conventional, and destructive. Hematoxylin and eosin (H&E) staining and CD31, desmin, and dual CD31âdesmin immunolabeling were performed. Foci number and patterns of VI were compared with the test and validation sets. More foci of VI were detected by single CD31 (P = 0.022) than H&E staining in the test set. CD31 immunolabeling detected more foci of the conventional pattern of VI, and desmin immunolabeling detected more foci of the destructive pattern (all, P < 0.001). Dual CD31âdesmin immunolabeling identified more foci of VI (P = 0.012) and specifically detected more foci of IN-like (P = 0.045) and destructive patterns (P < 0.001) than H&E staining in the validation set. However, dual CD31âdesmin immunolabeling was not helpful for detecting the conventional pattern of VI in the validation set. Patients with VI detected by dual CD31âdesmin immunolabeling had shorter disease-free survival (P <0.001) than those without VI. VI detected by dual CD31âdesmin immunolabeling was a worse prognostic indicator (P = 0.009). More foci of VI could be detected with additional single CD31 or dual CD31âdesmin immunolabeling. The precise evaluation of VI with dual CD31âdesmin immunolabeling can provide additional prognostic information for patients with surgically resected pancreatobiliary tract cancers.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Neoplasias Pancreáticas
/
Neoplasias del Sistema Biliar
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Molécula-1 de Adhesión Celular Endotelial de Plaqueta
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Desmina
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Invasividad Neoplásica
Tipo de estudio:
Prognostic_studies
Límite:
Aged
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Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2020
Tipo del documento:
Article