Evaluation of PR3- and MPO-ANCA line and dot immunoassays in ANCA-associated vasculitis.
Rheumatology (Oxford)
; 60(9): 4390-4394, 2021 09 01.
Article
en En
| MEDLINE
| ID: mdl-33316047
ABSTRACT
OBJECTIVE:
This study was performed to evaluate the diagnostic accuracy of novel line and dot immunoassays for detection of MPO and PR3 ANCA.METHODS:
Sera from 50 patients with ANCA-associated vasculitis (AAV), including granulomatosis with polyangiitis and microscopic polyangiitis, and from 45 disease controls were tested by IIF and for the presence of PR3-ANCA and MPO-ANCA by four different line or dot immunoassays, as well as by a chemiluminescence immunoassay.RESULTS:
The area under the curve of the receiver operating characteristic curve to discriminate AAV from controls was 0.858 (95% CI 0.785-0.931) for the IIF method. For the antigen-specific immunoassays, the area under the curve varied between 0.869 (95% CI 0.797-0.941) and 0.936 (95% 0.886-0.985).CONCLUSIONS:
Our comparison of various ANCA detection methods showed a high degree of diagnostic precision for all of the PR3- and MPO-ANCA line and dot immunoassays investigated. The performance was equal to or better than the performance of IIF. These results indicate that novel line and dot immunoassays can serve as a first-line test method in patients with the suspected diagnosis of AAV.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Anticuerpos Anticitoplasma de Neutrófilos
/
Vasculitis Asociada a Anticuerpos Citoplasmáticos Antineutrófilos
Tipo de estudio:
Diagnostic_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Límite:
Female
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Humans
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Male
Idioma:
En
Revista:
Rheumatology (Oxford)
Asunto de la revista:
REUMATOLOGIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Alemania