Generation of a miR-26b stem-loop knockout human iPSC line, MCRIi019-A-1, using CRISPR/Cas9 editing.

Kung, Louise H W; Sampurno, Lisa; Little, Christopher B; Lamandé, Shireen R; Bateman, John F

Kung, Louise H W; Sampurno, Lisa; Little, Christopher B; Lamandé, Shireen R; Bateman, John F.

Afiliación

Kung LHW; Murdoch Children's Research Institute, Parkville, Victoria, Australia.
Sampurno L; Murdoch Children's Research Institute, Parkville, Victoria, Australia.
Little CB; Raymond Purves Bone and Joint Research Laboratories, Kolling Institute of Medical Research, Institute of Bone and Joint Research, University of Sydney, St Leonards, New South Wales, Australia.
Lamandé SR; Murdoch Children's Research Institute, Parkville, Victoria, Australia; Department of Paediatrics, University of Melbourne, Australia.
Bateman JF; Murdoch Children's Research Institute, Parkville, Victoria, Australia; Department of Paediatrics, University of Melbourne, Australia. Electronic address: john.bateman@mcri.edu.au.

Stem Cell Res ; 50: 102118, 2020 Dec 10.

Article en En | MEDLINE | ID: mdl-33316599

RESUMEN

miR-26b has been implicated in a wide range of human diseases, including cancer, diabetes, heart disease, Alzheimer's disease and osteoarthritis. To provide a tool to explore the importance of miR-26b in this broad context, we have generated and characterized a homozygous miR-26b stem-loop knockout human iPSC line. This gene-edited line exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. This iPSC line will be valuable for studies investigating disease mechanisms and testing therapeutic strategies in vitro.

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Texto completo: 1 Bases de datos: MEDLINE Idioma: En Revista: Stem Cell Res Año: 2020 Tipo del documento: Article País de afiliación: Australia