SLPI in periodontal Ligament is not sleepy during biophysical force-induced tooth movement.
J Clin Periodontol
; 48(4): 528-540, 2021 04.
Article
en En
| MEDLINE
| ID: mdl-33370451
ABSTRACT
AIM:
We aimed to identify a key molecule that maintains periodontal tissue homeostasis during biophysical force-induced tooth movement (BTM) by orchestrating alveolar bone (AB) remodelling. MATERIALS ANDMETHODS:
Differential display-PCR was performed to identify key molecules for BTM in rats. To investigate the localization and expression of the identified molecules, immunofluorescence, real-time RT-PCR and Western blotting were performed in rats and human periodontal ligament (PDL) cells. Functional test and micro-CT analysis were performed to examine the in vivo effects of the identified molecules on BTM.RESULTS:
Secretory leucocyte peptidase inhibitor (SLPI) in the PDL was revealed as a key molecule for BTM-induced AB remodelling. SLPI was enhanced in the PDL under both compression and tension, and downregulated by an adenyl cyclases inhibitor. SLPI induced osteoblastogenic genes including runt-related transcription factor 2 (Runx2) and synergistically augmented tension-induced Runx2 expression. SLPI augmented mineralization in PDL cells. SLPI induced osteoclastogenic genes including receptor activator of nuclear factor kappa-Β ligand (RANKL) and synergistically augmented the compression-induced RANKL and macrophage colony-stimulating factor (MCSF) expression. Finally, the in vivo SLPI application into the AB significantly augmented BTM.CONCLUSIONS:
SLPI or its inhibitors might serve as a biological target molecule for therapeutic interventions to modulate BTM.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ligamento Periodontal
/
Ligando RANK
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Clin Periodontol
Año:
2021
Tipo del documento:
Article