Combined negative dielectrophoresis with a flexible SERS platform as a novel strategy for rapid detection and identification of bacteria.
Anal Bioanal Chem
; 413(7): 2007-2020, 2021 Mar.
Article
en En
| MEDLINE
| ID: mdl-33507352
ABSTRACT
Surface-enhanced Raman spectroscopy (SERS) is a vibrational method successfully applied in analytical chemistry, molecular biology and medical diagnostics. In this article, we demonstrate the combination of the negative dielectrophoretic (nDEP) phenomenon and a flexible surface-enhanced Raman platform for quick isolation (3 min), concentration and label-free identification of bacteria. The platform ensures a strong enhancement factor, high stability and reproducibility for the SERS response of analyzed samples. By introducing radial dielectrophoretic forces directed at the SERS platform, we can efficiently execute bacterial cell separation, concentration and deposition onto the SERS-active surface, which simultaneously works as a counter electrode and thus enables such hybrid DEP-SERS device vibration-based detection. Additionally, we show the ability of our DEP-SERS system to perform rapid, cultivation-free, direct detection of bacteria in urine and apple juice samples. The device provides new opportunities for the detection of pathogens.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Espectrometría Raman
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Bacteriuria
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Orina
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Electroforesis
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Jugos de Frutas y Vegetales
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Microbiología de Alimentos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2021
Tipo del documento:
Article
País de afiliación:
Polonia