Conditional gene silencing via a CRISPR system in cerebellar Purkinje cells.
Biochim Biophys Acta Gen Subj
; 1865(6): 129869, 2021 06.
Article
en En
| MEDLINE
| ID: mdl-33581252
ABSTRACT
⢠Specific gene knockdown in cerebellar Purkinje cells is a major challenge because Purkinje cells only make up a small fraction of the cerebellum and off-target effects of shRNA occur. ⢠Little is known about the application of CRISPR-Cas13 and the resulting protein expression levels in Purkinje cells, a type of developing postmitotic neuron. ⢠We explored the possibility of a Cas13-mediated conditional knockdown system for cerebellar Purkinje cells. This system can achieve a suppression of the target proteins restricted to Purkinje cells in mixed cerebellar cultures.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Células de Purkinje
/
Proteína Quinasa C
/
Cerebelo
/
Silenciador del Gen
/
Sistemas CRISPR-Cas
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta Gen Subj
Año:
2021
Tipo del documento:
Article