Advances in sulfur mustard-induced DNA adducts: Characterization and detection.
Toxicol Lett
; 344: 46-57, 2021 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-33705862
ABSTRACT
Sulfur mustard (SM) is a blister chemical warfare agent with severe cytotoxicity and genotoxicity. It can extensively alkylate important macromolecules in organisms, such as proteins, DNA, and lipids, and produce a series of metabolites, among which the characteristic ones can be used as biomarkers. The exact toxicological mechanisms of SM remain unclear but mainly involve the DNA lesions induced by alkylation and oxidative stress caused by glutathione depletion. Various methods have been used to analyze DNA damage caused by SM. Among these methods, liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology stands out and makes it possible to observe damage in view of biomarkers induced by SM. Sample preparation is critical for detection by LC-MS/MS and mainly includes DNA isolation, adduct hydrolysis, and adduct purification. Moreover, optimization of chromatographic conditions, selection of MS transitions, and quantitative strategies are also essential. SM-DNA adducts are generally considered to be N7-HETEG, O6-HETEG, N7-BisG, and N3-HETEA. This article proposes some other possibilities of SM-DNA adducts for the identification of SM genotoxicity.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Sustancias para la Guerra Química
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Aductos de ADN
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Gas Mostaza
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
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Humans
Idioma:
En
Revista:
Toxicol Lett
Año:
2021
Tipo del documento:
Article