Modeling human hepatic steatosis in pluripotent stem cell-derived hepatocytes.
STAR Protoc
; 2(2): 100493, 2021 06 18.
Article
en En
| MEDLINE
| ID: mdl-33997813
ABSTRACT
This protocol describes the production of hepatocyte-like cells (HLCs) from human pluripotent stem cells and how to induce hepatic steatosis, a condition characterized by intracellular lipid accumulation. Following differentiation to an HLC phenotype, intracellular lipid accumulation is induced with a steatosis induction cocktail, allowing the user to examine the cellular processes that underpin hepatic steatosis. Furthermore, the renewable nature of our system, on a defined genetic background, permits in-depth mechanistic analysis, which may facilitate therapeutic target identification in the future. For complete details on the use and execution of this protocol, please refer to Sinton et al. (2021).
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
/
Hepatocitos
/
Células Madre Pluripotentes
/
Hígado Graso
/
Modelos Biológicos
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2021
Tipo del documento:
Article
País de afiliación:
Reino Unido