Phenotypic and Functional Heterogeneity of Low-Density and High-Density Human Lung Macrophages.
Biomedicines
; 9(5)2021 May 04.
Article
en En
| MEDLINE
| ID: mdl-34064389
ABSTRACT
BACKGROUND:
Pulmonary macrophages are a highly heterogeneous cell population distributed in different lung compartments.METHODS:
We separated two subpopulations of macrophages from human lung parenchyma according to flotation over density gradients.RESULTS:
Two-thirds 65.4% of the lung macrophages have a density between 1.065 and 1.078 (high-density macrophages HDMs), and the remaining one-third (34.6) had a density between 1.039 and 1.052 (low-density macrophages LDMs). LDMs had a larger area (691 vs. 462 µm2) and cell perimeter (94 vs. 77 µm) compared to HDMs. A significantly higher percentage of HDMs expressed CD40, CD45, and CD86 compared to LDMs. In contrast, a higher percentage of LDMs expressed the activation markers CD63 and CD64. The release of TNF-α, IL-6, IL-10 and IL-12 induced by lipopolysaccharide (LPS) was significantly higher in HDMs than in LDMs.CONCLUSION:
The human lung contains two subpopulations of macrophages that differ in buoyancy, morphometric parameters, surface marker expression and response to LPS. These subpopulations of macrophages probably play distinct roles in lung inflammation and immune responses.
Texto completo:
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Bases de datos:
MEDLINE
Idioma:
En
Revista:
Biomedicines
Año:
2021
Tipo del documento:
Article
País de afiliación:
Italia