A single cell characterisation of human embryogenesis identifies pluripotency transitions and putative anterior hypoblast centre.
Nat Commun
; 12(1): 3679, 2021 06 17.
Article
en En
| MEDLINE
| ID: mdl-34140473
ABSTRACT
Following implantation, the human embryo undergoes major morphogenetic transformations that establish the future body plan. While the molecular events underpinning this process are established in mice, they remain unknown in humans. Here we characterise key events of human embryo morphogenesis, in the period between implantation and gastrulation, using single-cell analyses and functional studies. First, the embryonic epiblast cells transition through different pluripotent states and act as a source of FGF signals that ensure proliferation of both embryonic and extra-embryonic tissues. In a subset of embryos, we identify a group of asymmetrically positioned extra-embryonic hypoblast cells expressing inhibitors of BMP, NODAL and WNT signalling pathways. We suggest that this group of cells can act as the anterior singalling centre to pattern the epiblast. These results provide insights into pluripotency state transitions, the role of FGF signalling and the specification of anterior-posterior axis during human embryo development.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Implantación del Embrión
/
Regulación del Desarrollo de la Expresión Génica
/
Desarrollo Embrionario
/
Gastrulación
/
Análisis de la Célula Individual
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Vía de Señalización Wnt
/
Estratos Germinativos
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Reino Unido