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Unique anticodon loop conformation with the flipped-out wobble nucleotide in the crystal structure of unbound tRNAVal.
Jeong, Hyeonju; Kim, Jungwook.
Afiliación
  • Jeong H; Department of Chemistry, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
  • Kim J; Department of Chemistry, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
RNA ; 27(11): 1330-1338, 2021 11.
Article en En | MEDLINE | ID: mdl-34315814
During protein synthesis on ribosome, tRNA recognizes its cognate codon of mRNA through base-pairing with the anticodon. The 5'-end nucleotide of the anticodon is capable of wobble base-pairing, offering a molecular basis for codon degeneracy. The wobble nucleotide is often targeted for post-transcriptional modification, which affects the specificity and fidelity of the decoding process. Flipping-out of a wobble nucleotide in the anticodon loop has been proposed to be necessary for modifying enzymes to access the target nucleotide, which has been captured in selective structures of protein-bound complexes. Meanwhile, all other structures of free or ribosome-bound tRNA display anticodon bases arranged in stacked conformation. We report the X-ray crystal structure of unbound tRNAVal1 to a 2.04 Å resolution showing two different conformational states of wobble uridine in the anticodon loop, one stacked on the neighboring base and the other swiveled out toward solvent. In addition, the structure reveals a rare magnesium ion coordination to the nitrogen atom of a nucleobase, which has been sampled very rarely among known structures of nucleic acids.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Ribosomas / Biosíntesis de Proteínas / Anticodón / ARN Mensajero / ARN de Transferencia de Valina Idioma: En Revista: RNA Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Ribosomas / Biosíntesis de Proteínas / Anticodón / ARN Mensajero / ARN de Transferencia de Valina Idioma: En Revista: RNA Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article