Obtaining High-Purity Docosahexaenoic Acid Oil in Thraustochytrid Aurantiochytrium through a Combined Metabolic Engineering Strategy.
J Agric Food Chem
; 69(35): 10215-10222, 2021 Sep 08.
Article
en En
| MEDLINE
| ID: mdl-34415758
ABSTRACT
High-purity docosahexaenoic acid (DHA) resources are insufficient in the pharmaceutical and food industries. Although many efforts have attempted to obtain the high-purity DHA production, few reports have been successful. Here, a combined metabolic engineering strategy was employed to increase the DHA purity in the oleaginous thraustochytrid Aurantiochytrium. The strategy includes both partial deactivation of the competing pathway of DHA biosynthesis, by disrupting one copy of the fatty acid synthase gene, and strengthening of substrate supply and triacylglycerol synthesis, by the overexpression of acetyl-CoA carboxylase and diacylglycerol acyltransferase. With this strategy, a final mutant was obtained with a DHA purity of 61% in total fatty acids and a content of 331 mg/g dry cell weight. This study provides an advanced strategy for sustainable high-purity DHA production and highlights the strategy for producing designer oils in industrial oleaginous microorganisms.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ácidos Docosahexaenoicos
/
Estramenopilos
Idioma:
En
Revista:
J Agric Food Chem
Año:
2021
Tipo del documento:
Article