Kinetic Analysis of a Protein-protein Complex to Determine its Dissociation Constant (K<sub>D</sub>) and the Effective Concentration (EC<sub>50</sub>) of an Interplaying Effector Molecule Using Bio-layer Interferometry.

Orthwein, Tim; Huergo, Luciano F; Forchhammer, Karl; Selim, Khaled A

Kinetic Analysis of a Protein-protein Complex to Determine its Dissociation Constant (K_D) and the Effective Concentration (EC₅₀) of an Interplaying Effector Molecule Using Bio-layer Interferometry.

Orthwein, Tim; Huergo, Luciano F; Forchhammer, Karl; Selim, Khaled A.

Afiliación

Orthwein T; Organismic Interactions Department, Interfaculty Institute for Microbiology and Infection Medicine, Cluster of Excellence 'Controlling Microbes to Fight Infections', Tübingen University, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
Huergo LF; Setor Litoral, Federal University of Paraná (UFPR), Matinhos, Brazil.
Forchhammer K; Organismic Interactions Department, Interfaculty Institute for Microbiology and Infection Medicine, Cluster of Excellence 'Controlling Microbes to Fight Infections', Tübingen University, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
Selim KA; Organismic Interactions Department, Interfaculty Institute for Microbiology and Infection Medicine, Cluster of Excellence 'Controlling Microbes to Fight Infections', Tübingen University, Auf der Morgenstelle 28, 72076 Tübingen, Germany.

Bio Protoc ; 11(17): e4152, 2021 Sep 05.

Article en En | MEDLINE | ID: mdl-34604457

Palabras clave

EC50; IC50; KD; Binding kinetics; Biolayer interferometry; PII signaling protein; Protein-protein interaction

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Texto completo: 1 Bases de datos: MEDLINE Idioma: En Revista: Bio Protoc Año: 2021 Tipo del documento: Article País de afiliación: Alemania