Next-generation sequencing confirms T-cell clonality in a subset of pediatric pityriasis lichenoides.
J Cutan Pathol
; 49(3): 252-260, 2022 Mar.
Article
en En
| MEDLINE
| ID: mdl-34614220
ABSTRACT
BACKGROUND:
Pityriasis lichenoides (PL) is a papulosquamous disease that affects both adults and children. Previous studies have shown a subset of this entity to have clonal T-cell populations via PCR-based assays. In this study, we sought to implement next-generation sequencing (NGS) as a more sensitive and specific test to examine for T-cell clonality within the pediatric population.METHODS:
We identified 18 biopsy specimens from 12 pediatric patients with clinical and histopathologic findings compatible with PL. Patient demographics, clinical features, management, and histopathologic findings were reviewed. All specimens were analyzed for clonality with NGS of T-cell receptor beta (TRB) and gamma (TRG) genes.RESULTS:
Of the 12 patients, 9 (75%) had complete resolution of lesions at the time of data collection (mean follow-up 31 months). The remaining three patients significantly improved with methotrexate (with or without acitretin). Interestingly, 7 of 12 patients (58%) and 9 of 17 biopsy specimens (53%) showed evidence of T-cell clonality. Two patients showed matching TRB clones from different anatomic sites.CONCLUSIONS:
T-cell clonality is a common finding in PL, probably representing a "reactive clonality" rather than a true lymphoproliferative disorder. Clonality alone cannot be used as a means to distinguish PL from lymphomatoid papulosis or cutaneous lymphoma.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Clonación Molecular
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Pitiriasis Liquenoide
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Genes Codificadores de la Cadena beta de los Receptores de Linfocito T
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Genes Codificadores de la Cadena gamma de los Receptores de Linfocito T
Tipo de estudio:
Prognostic_studies
Límite:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Male
Idioma:
En
Revista:
J Cutan Pathol
Año:
2022
Tipo del documento:
Article
País de afiliación:
Estados Unidos