Co-targeting strategy for precise, scarless gene editing with CRISPR/Cas9 and donor ssODNs in Chlamydomonas.

Akella, Soujanya; Ma, Xinrong; Bacova, Romana; Harmer, Zachary P; Kolackova, Martina; Wen, Xiaoxue; Wright, David A; Spalding, Martin H; Weeks, Donald P; Cerutti, Heriberto

Akella, Soujanya; Ma, Xinrong; Bacova, Romana; Harmer, Zachary P; Kolackova, Martina; Wen, Xiaoxue; Wright, David A; Spalding, Martin H; Weeks, Donald P; Cerutti, Heriberto.

Afiliación

Akella S; School of Biological Sciences and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.
Ma X; School of Biological Sciences and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.
Bacova R; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic.
Harmer ZP; School of Biological Sciences and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.
Kolackova M; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic.
Wen X; School of Biological Sciences and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.
Wright DA; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
Spalding MH; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
Weeks DP; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.
Cerutti H; School of Biological Sciences and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.

Plant Physiol ; 187(4): 2637-2655, 2021 12 04.

Article en En | MEDLINE | ID: mdl-34618092

Asunto(s)

Proteínas Algáceas/genética; Sistemas CRISPR-Cas; Chlamydomonas/genética; Edición Génica/métodos; Ribonucleoproteínas/genética

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Ribonucleoproteínas / Chlamydomonas / Proteínas Algáceas / Sistemas CRISPR-Cas / Edición Génica Idioma: En Revista: Plant Physiol Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos

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