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Spatiotemporal expression of long noncoding RNA Moshe modulates heart cell lineage commitment.
Kim, Na-Jung; Lee, Kang-Hoon; Son, YeonSung; Nam, A-Reum; Moon, Eun-Hye; Pyun, Jung-Hoon; Park, Jinyoung; Kang, Jong-Sun; Lee, Young Jae; Cho, Je-Yoel.
Afiliación
  • Kim NJ; Department of Biochemistry, BK21 Plus and Research Institute for Veterinary Science, School of Veterinary Medicine, Seoul National University, Seoul, Korea.
  • Lee KH; Department of Biochemistry, BK21 Plus and Research Institute for Veterinary Science, School of Veterinary Medicine, Seoul National University, Seoul, Korea.
  • Son Y; Department of Biochemistry, BK21 Plus and Research Institute for Veterinary Science, School of Veterinary Medicine, Seoul National University, Seoul, Korea.
  • Nam AR; Department of Biochemistry, BK21 Plus and Research Institute for Veterinary Science, School of Veterinary Medicine, Seoul National University, Seoul, Korea.
  • Moon EH; Lee Gil Ya Cancer and Diabetes Institute, Department of Biochemistry, Gachon University, Yeonsu-gu, Republic of Korea.
  • Pyun JH; Department of Molecular Cell Biology, Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon, Korea.
  • Park J; Department of Biochemistry, School of Pharmacy, Sungkyunkwan University, Suwon, Republic of Korea.
  • Kang JS; Department of Molecular Cell Biology, Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon, Korea.
  • Lee YJ; Lee Gil Ya Cancer and Diabetes Institute, Department of Biochemistry, Gachon University, Yeonsu-gu, Republic of Korea.
  • Cho JY; Department of Biochemistry, BK21 Plus and Research Institute for Veterinary Science, School of Veterinary Medicine, Seoul National University, Seoul, Korea.
RNA Biol ; 18(sup2): 640-654, 2021 11 12.
Article en En | MEDLINE | ID: mdl-34755591
The roles of long non-coding RNA (LncRNA) have been highlighted in various development processes including congenital heart defects (CHD). Here, we characterized the molecular function of LncRNA, Moshe (1010001N08ik-203), one of the Gata6 antisense transcripts located upstream of Gata6, which is involved in both heart development and the most common type of congenital heart defect, atrial septal defect (ASD). During mouse embryonic development, Moshe was first detected during the cardiac mesoderm stage (E8.5 to E9.5) where Gata6 is expressed and continues to increase at the atrioventricular septum (E12.5), which is involved in ASD. Functionally, the knock-down of Moshe during cardiogenesis caused significant repression of Nkx2.5 in cardiac progenitor stages and resulted in the increase in major SHF lineage genes, such as cardiac transcriptional factors (Isl1, Hand2, Tbx2), endothelial-specific genes (Cd31, Flk1, Tie1, vWF), a smooth muscle actin (a-Sma) and sinoatrial node-specific genes (Shox2, Tbx18). Chromatin Isolation by RNA Purification showed Moshe activates Nkx2.5 gene expression via direct binding to its promoter region. Of note, Moshe was conserved across species, including human, pig and mouse. Altogether, this study suggests that Moshe is a heart-enriched lncRNA that controls a sophisticated network of cardiogenesis by repressing genes in SHF via Nkx2.5 during cardiac development and may play an important role in ASD.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Diferenciación Celular / Linaje de la Célula / Miocitos Cardíacos / ARN Largo no Codificante Límite: Animals / Humans Idioma: En Revista: RNA Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Diferenciación Celular / Linaje de la Célula / Miocitos Cardíacos / ARN Largo no Codificante Límite: Animals / Humans Idioma: En Revista: RNA Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article