Assessment of the effect of kinetin against formic acid toxicity in chicken embryo model.

ABSTRACT

The current study was designed to investigate the in ovo injection of formic acid (FA) on hatchability rate (HR; Experiment 1) and the potential ameliorative role of kinetin concurrent with FA on biochemical parameters of hatched broilers (Experiment 2). In Experiment 1, live embryonated eggs (n = 280; Day 4 of incubation) were in ovo injected with 0.03, 0.06, 0.125, 0.25, 0.50, 1, 2, 4, 8, 16 and 32 m m FA. In Experiment 2, intra-yolk-sac administration of toxic doses of FA (2 m m) concurrent with kinetin at 50, 100 or 200 µ m were evaluated on hatched embryos. The amount of malondialdehyde (MDA), total antioxidant capacity (TAC), total nitrate-nitrite (TNN), total lipid hydroperoxide (TLHP) and superoxide dismutase (SOD) activity was measured in serum, liver, heart and brain tissues. The results revealed that injection of 2 mM FA significantly increased mortality compared to the control group (p < 0.05). Concurrent administration of 50 or 100 µ m kinetin + 2 m m FA increased HR to 10% and 20% compared to the FA-alone-treated group, respectively. Intra-yolk-sac-received FA group showed greater amounts of MDA, TLHP and TNN and lesser amounts of TAC and SOD activity in serum and tissue samples of liver, heart and brain compared to control groups (p < 0.001). In comparison to the FA-alone-treated group, all doses of kinetin were able to increase the TAC levels in serum and tissue samples when administered concurrently with FA. The doses of 50 and 100 µ m kinetin were efficacious to ameliorate the toxic role of FA injection on SOD activities (p < 0.001). Co-injection of 100 µ m kinetin plus FA significantly reduced the amounts of MDA, TNN and TLHP in measured samples compared to the FA-alone-injected group (p < 0.001). Our results indicated that kinetin (especially at 100 µ m doses) would ameliorate the toxic effects of FA on developing live chicken embryos.