Fruitflow inhibits platelet function by suppressing Akt/GSK3ß, Syk/PLCγ2 and p38 MAPK phosphorylation in collagen-stimulated platelets.
BMC Complement Med Ther
; 22(1): 75, 2022 Mar 17.
Article
en En
| MEDLINE
| ID: mdl-35300669
ABSTRACT
BACKGROUND:
Platelets play an important role in the progression of atherosclerosis and cardiovascular events. The inhibition of platelet function is a main strategy to reduce risk of cardiovascular events. Some studies have shown that tomato extracts inhibit platelet function, but the molecular mechanisms remain unclear. Fruitflow is a water-solute tomato extract and the main ingredients including flavonoids, adenosine, chlorogenic acid, phytosterols, naringenin, and carotenoids. The present study investigated the effects of fruitflow on adenosine diphosphate (ADP)- and collagen- stimulated platelet aggregation, platelet adhesion, and levels of thromboxane B2 (TXB2), 6-keto-prostaglandin F1α (PGF1α), and platelet factor 4 (PF4) and explored the underlying molecular mechanisms.METHODS:
Platelet-rich plasma (PRP) was used for measurement of platelet aggregation, TXB2, 6-keto- PGF1α, and PF4 levels. Platelet aggregation was analyzed using a Chrono-Log aggregometer. TXB2, 6-keto- PGF1α, and PF4 levels were determined using enzyme-linked immunosorbent assay kits. Immunoblotting was used to detect protein expression and phosphorylation on washed platelets. Platelet adhesion and spreading were determined by immunofluorescence.RESULTS:
Fruitflow (1, 3, 10 and 100 µg/ml) dose-dependently inhibited platelet aggregation that was induced by ADP and collagen. Fruitflow (100 µg/ml) treatment completely suppressed ADP- and collagen-stimulated platelet aggregation. Fruitflow (100 µg/ml) significantly decreased TXB2 and 6-keto-PGF1α generation and PF4 release in ADP- and collagen-stimulated platelets. Treatment with fruitflow effectively blocked collagen-induced platelet spreading. To determine the potential molecule mechanism of action of fruitflow, we investigated the protein expression and phosphorylation of several signaling molecules in collagen-activated platelets. Fruitflow dose-dependently suppressed Akt, Glycogen synthase kinase-3ß (GSK-3ß), spleen tyrosine kinase (Syk) and phospholipase Cγ2 (PLCγ2) and p38 MAPK phosphorylation that was induced by collagen.CONCLUSION:
Fruitflow inhibited platelet aggregation and reduced TXB2, 6-keto-PGF1α, and PF4 levels in ADP- and collagen-stimulated platelets. The mechanism of action of fruitflow may be associated with the suppression of Akt/GSK3ß, Syk/PLCγ2, and p38 MAPK phosphorylation in collagen-activated platelets. Fruitflow is a natural product derived from tomato and can be used as a health food for decreasing platelet activity.Palabras clave
Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Plaquetas
/
Proteínas Proto-Oncogénicas c-akt
Idioma:
En
Revista:
BMC Complement Med Ther
Año:
2022
Tipo del documento:
Article