Development of SSR and SNP markers for identifying opium poppy.
Int J Legal Med
; 136(5): 1261-1271, 2022 Sep.
Article
en En
| MEDLINE
| ID: mdl-35316386
ABSTRACT
Papaver somniferum L. (opium poppy) is the original plant of heroin, which is a major narcotic drug, and this plant has brought great harm to human health. However, the ban on opium poppy cultivation and trafficking is facing great challenges because of abnormal profits. Therefore, rapid and accurate identification is important to address the abovementioned problems. In this study, eleven simple sequence repeats (SSR) markers and two single nucleotide polymorphism (SNP) markers were mined to distinguish opium poppy from other six Papaver species. These molecular markers were further verified through a large number of plant materials of these seven Papaver species. An excellent multiplex polymerase chain reaction (PCR) system that simultaneously amplifies the three of eleven SSR markers was developed, which effectively improves the efficiency and speed of identification. The present research is of great implication for identifying and investigating the illegal cultivation and trafficking of opium poppy.
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Bases de datos:
MEDLINE
Asunto principal:
Papaver
Idioma:
En
Revista:
Int J Legal Med
Asunto de la revista:
JURISPRUDENCIA
Año:
2022
Tipo del documento:
Article