Establishment of four head and neck squamous cell carcinoma cell lines: importance of reference DNA for accurate genomic characterisation.
J Laryngol Otol
; 137(3): 301-307, 2023 Mar.
Article
en En
| MEDLINE
| ID: mdl-35317874
ABSTRACT
OBJECTIVE:
There is significant interest in developing early passage cell lines with matched normal reference DNA to facilitate a precision medicine approach in assessing drug response. This study aimed to establish early passage cell lines, and perform whole exome sequencing and short tandem repeat profiling on matched normal reference DNA, primary tumour and corresponding cell lines.METHODS:
A cell culture based, in vitro study was conducted of patients with primary human papillomavirus positive and human papillomavirus negative tumours.RESULTS:
Four early passage cell lines were established. Two cell lines were human papillomavirus positive, confirmed by sequencing and p16 immunoblotting. Short tandem repeat profiling confirmed that all cell lines were established from their index tumours. Whole exome sequencing revealed that the matched normal reference DNA was critical for accurate mutationalanalysis:
a high rate of false positive mutation calls were excluded (87.6 per cent).CONCLUSION:
Early passage cell lines were successfully established. Patient-matched reference DNA is important for accurate cell line mutational calls.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Carcinoma de Células Escamosas
/
Infecciones por Papillomavirus
/
Neoplasias de Cabeza y Cuello
Límite:
Humans
Idioma:
En
Revista:
J Laryngol Otol
Asunto de la revista:
OTORRINOLARINGOLOGIA
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos