A gold nanoparticle-based lateral flow immunoassay for atrazine point-of-care detection using a handhold scanning device as reader.
Mikrochim Acta
; 189(4): 153, 2022 03 23.
Article
en En
| MEDLINE
| ID: mdl-35322310
ABSTRACT
A method is described to achieve accurate quantitative detection of atrazine (ATZ) in maize by using lateral flow strips based on gold nanoparticles (GNPs) and a handheld scanning reader. GNPs of 15 nm in diameter were applied as label, and a lateral flow immune assay strip was prepared. The linear range was 5.01-95.86 ng mL-1 with a detection limit of 4.92 ng mL-1 in phosphate buffer, 4 times better than the readout by the naked eye. ATZ-spiked corn samples were also analysed. The accuracy of results of spiked samples was confirmed by ELISA and liquid chromatography-tandem mass spectrometry (HPLC), which proved the reliability of the proposed method. A handhold device with an optical scanning system was designed for on-site quantitative detection. Combined with the pretreatment, the assay could be completed in less than 20 min.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Atrazina
/
Nanopartículas del Metal
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Mikrochim Acta
Año:
2022
Tipo del documento:
Article
País de afiliación:
China