NEW EXPERIMENTAL APPROACH IN BIODOSIMETRY: EX VIVO APOPTOSIS DETECTION.
Radiat Prot Dosimetry
; 198(9-11): 590-596, 2022 Aug 22.
Article
en En
| MEDLINE
| ID: mdl-36005967
ABSTRACT
This study establishes a new experimental approach for retrospective biodosimetric assessment by apoptosis detection ex vivo. For this purpose, we used mononuclear blood leukocytes isolated from the peripheral blood of irradiated Wistar rats and cultured them ex vivo for posterior analysis. Using flow cytometry, we distinguished apoptotic lymphocyte subsets individual biodosimetric potential at different time periods after exposure B-lymphocytes 6-8 h (0-7 Gy), natural killer cells 24 h (0-7 Gy) and T-lymphocytes 24 h (0-1 Gy). This novel experimental design innovates through the need of a single blood sample from irradiated individuals for a complete biodosimetric assessment.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Subgrupos Linfocitarios
/
Apoptosis
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
Límite:
Animals
Idioma:
En
Revista:
Radiat Prot Dosimetry
Año:
2022
Tipo del documento:
Article
País de afiliación:
República Checa