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Mapping monoclonal anti-SARS-CoV-2 antibody repertoires against diverse coronavirus antigens.
de Souza, Matheus Oliveira; Madan, Bharat; Teng, I-Ting; Huang, Aric; Liu, Lihong; Fahad, Ahmed S; Lopez Acevedo, Sheila N; Pan, Xiaoli; Sastry, Mallika; Gutierrez-Gonzalez, Matias; Yin, Michael T; Zhou, Tongqing; Ho, David D; Kwong, Peter D; DeKosky, Brandon J.
Afiliación
  • de Souza MO; Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS, United States.
  • Madan B; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.
  • Teng IT; Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS, United States.
  • Huang A; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.
  • Liu L; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD, United States.
  • Fahad AS; Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS, United States.
  • Lopez Acevedo SN; Aaron Diamond acquired immunodeficiency syndrome (AIDS) Research Center, Columbia University Irving Medical Center, New York, NY, United States.
  • Pan X; Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS, United States.
  • Sastry M; Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS, United States.
  • Gutierrez-Gonzalez M; Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS, United States.
  • Yin MT; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD, United States.
  • Zhou T; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.
  • Ho DD; Department of Medicine , Division of Infectious Diseases, Columbia University Irving Medical Center, New York, NY, United States.
  • Kwong PD; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD, United States.
  • DeKosky BJ; Aaron Diamond acquired immunodeficiency syndrome (AIDS) Research Center, Columbia University Irving Medical Center, New York, NY, United States.
Front Immunol ; 13: 977064, 2022.
Article en En | MEDLINE | ID: mdl-36119018
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged continuously, challenging the effectiveness of vaccines, diagnostics, and treatments. Moreover, the possibility of the appearance of a new betacoronavirus with high transmissibility and high fatality is reason for concern. In this study, we used a natively paired yeast display technology, combined with next-generation sequencing (NGS) and massive bioinformatic analysis to perform a comprehensive study of subdomain specificity of natural human antibodies from two convalescent donors. Using this screening technology, we mapped the cross-reactive responses of antibodies generated by the two donors against SARS-CoV-2 variants and other betacoronaviruses. We tested the neutralization potency of a set of the cross-reactive antibodies generated in this study and observed that most of the antibodies produced by these patients were non-neutralizing. We performed a comparison of the specific and non-specific antibodies by somatic hypermutation in a repertoire-scale for the two individuals and observed that the degree of somatic hypermutation was unique for each patient. The data from this study provide functional insights into cross-reactive antibodies that can assist in the development of strategies against emerging SARS-CoV-2 variants and divergent betacoronaviruses.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Límite: Humans Idioma: En Revista: Front Immunol Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Límite: Humans Idioma: En Revista: Front Immunol Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos