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Glial cell response to constant low light exposure in rat retina.
Bruera, Manuel G; Benedetto, María M; Guido, Mario E; Degano, Alicia L; Contin, María A.
Afiliación
  • Bruera MG; Departamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
  • Benedetto MM; Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina.
  • Guido ME; Departamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
  • Degano AL; Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina.
  • Contin MA; Departamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Vis Neurosci ; 39: E005, 2022 09 27.
Article en En | MEDLINE | ID: mdl-36164752
To study the macroglia and microglia and the immune role in long-time light exposure in rat eyes, we performed glial cell characterization along the time-course of retinal degeneration induced by chronic exposure to low-intensity light. Animals were exposed to light for periods of 2, 4, 6, or 8 days, and the retinal glial response was evaluated by immunohistochemistry, western blot and real-time reverse transcription polymerase chain reaction. Retinal cells presented an increased expression of the macroglia marker GFAP, as well as increased mRNA levels of microglia markers Iba1 and CD68 after 6 days. Also, at this time-point, we found a higher number of Iba1-positive cells in the outer nuclear layer area; moreover, these cells showed the characteristic activated-microglia morphology. The expression levels of immune mediators TNF, IL-6, and chemokines CX3CR1 and CCL2 were also significantly increased after 6 days. All the events of glial activation occurred after 5-6 days of constant light exposure, when the number of photoreceptor cells has already decreased significantly. Herein, we demonstrated that glial and immune activation are secondary to neurodegeneration; in this scenario, our results suggest that photoreceptor death is an early event that occurs independently of glial-derived immune responses.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Traumatismos por Radiación / Retina / Degeneración Retiniana / Neuroglía / Interleucina-6 Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: Vis Neurosci Asunto de la revista: NEUROLOGIA / OFTALMOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Argentina

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Traumatismos por Radiación / Retina / Degeneración Retiniana / Neuroglía / Interleucina-6 Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: Vis Neurosci Asunto de la revista: NEUROLOGIA / OFTALMOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Argentina