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N-Hydroxy pipecolic acid methyl ester is involved in Arabidopsis immunity.
Mohnike, Lennart; Huang, Weijie; Worbs, Brigitte; Feussner, Kirstin; Zhang, Yuelin; Feussner, Ivo.
Afiliación
  • Mohnike L; University of Goettingen, Albrecht-von-Haller-Institute for Plant Sciences, Department of Plant Biochemistry, D-37077 Goettingen, Germany.
  • Huang W; University of British Columbia, Department of Botany, V6T 1Z4 Vancouver (BC), Canada.
  • Worbs B; University of Goettingen, Institute for Organic and Biomolecular Chemistry, Department of Organic Chemistry, D-37077 Goettingen, Germany.
  • Feussner K; University of Goettingen, Albrecht-von-Haller-Institute for Plant Sciences, Department of Plant Biochemistry, D-37077 Goettingen, Germany.
  • Zhang Y; University of Goettingen, Goettingen Center for Molecular Biosciences (GZMB), Service Unit for Metabolomics and Lipidomics, D-37077 Goettingen, Germany.
  • Feussner I; University of British Columbia, Department of Botany, V6T 1Z4 Vancouver (BC), Canada.
J Exp Bot ; 74(1): 458-471, 2023 01 01.
Article en En | MEDLINE | ID: mdl-36260503
The biosynthesis of N-hydroxy pipecolic acid (NHP) has been intensively studied, though knowledge on its metabolic turnover is still scarce. To close this gap, we discovered three novel metabolites via metabolite fingerprinting in Arabidopsis thaliana leaves after Pseudomonas infection and UV-C treatment. Exact mass information and fragmentation by tandem mass spectrometry (MS/MS) suggest a methylated derivative of NHP (MeNHP), an NHP-OGlc-hexosyl conjugate (NHP-OGlc-Hex), and an additional NHP-OGlc-derivative. All three compounds were formed in wild-type leaves but were not present in the NHP-deficient mutant fmo1-1. The identification of these novel NHP-based molecules was possible by a dual-infiltration experiment using a mixture of authentic NHP and D9-NHP standards for leaf infiltration followed by UV-C treatment. Interestingly, the signal intensity of MeNHP and other NHP-derived metabolites increased in ugt76b1-1 mutant plants. For MeNHP, we unequivocally determined the site of methylation at the carboxylic acid moiety. MeNHP application by leaf infiltration leads to the detection of a MeNHP-OGlc as well as NHP, suggesting MeNHP hydrolysis to NHP. This is in line with the observation that MeNHP infiltration is able to rescue the fmo1-1 susceptible phenotype against Hyaloperonospora arabidopsidis Noco 2. Together, these data suggest MeNHP as an additional storage or transport form of NHP.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis Tipo de estudio: Guideline Idioma: En Revista: J Exp Bot Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis Tipo de estudio: Guideline Idioma: En Revista: J Exp Bot Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article País de afiliación: Alemania