Effects of intra-articular injection of an acellular equine liquid amniotic allograft in healthy equine joints.
Vet Surg
; 52(1): 62-68, 2023 Jan.
Article
en En
| MEDLINE
| ID: mdl-36408850
ABSTRACT
OBJECTIVE:
Evaluate effects of acellular equine liquid amnion allograft (ELAA) injected into healthy equine joints. STUDYDESIGN:
Randomized, blinded, controlled experiment. ANIMALS Eight healthy adult horses.METHODS:
One intercarpal joint (ICJ) of each horse was randomly assigned to be injected with 1.5 ml of ELAA (treatment) while the contralateral ICJ was injected with 1.5 ml of 0.9% NaCl (control). Subjective lameness evaluation, force plate analysis, and synovial fluid analysis, including interleukin-1 receptor antagonist (IL-1ra) analysis, were performed before (day 0) and at days 1, 3, 5, and 10. Synovial fluid analysis was also performed on days 20 and 30.RESULTS:
No difference in subjective lameness (P = .75) and no decrease in peak vertical force or vertical impulse were seen in any limb on any day. Total nucleated cell count (TNCC) was increased in treatment joints on days 1 (P = .0007; T 6039 cells/µl, C 240 cells/µl) and 3 (P < .0001; T 1119 cells/µl, C 240 cells/µl). Log-10 transformed values for IL-1ra were higher in treated joints on days 1 (P = .0005; T 3553.7 pg/ml, C 1890.1 pg/ml) and 3 (P = .01; T 2283.2 pg/ml, C 1250.7 pg/ml).CONCLUSION:
Injection of ELAA into the ICJ caused an increase in synovial fluid TNCC in comparison with saline control but no lameness was observed. There was increased IL-1ra on days 1 and 3 after ELAA injection. CLINICALSIGNIFICANCE:
Intra-articular injection of ELAA into healthy equine joints results in no significant safety concerns. The observed increase in IL-1ra may provide beneficial effects in inflamed joints.
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Bases de datos:
MEDLINE
Asunto principal:
Proteína Antagonista del Receptor de Interleucina 1
/
Enfermedades de los Caballos
Tipo de estudio:
Clinical_trials
Límite:
Animals
Idioma:
En
Revista:
Vet Surg
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos