Whole-Genome Sequencing of a Multidrug-Resistant Strain: Delftia acidovorans B408.
Biochem Genet
; 61(3): 1086-1096, 2023 Jun.
Article
en En
| MEDLINE
| ID: mdl-36451050
ABSTRACT
BACKGROUND:
Delftia acidovorans is distributed widely in the environment and has the potential to promote the growth of plants and degrade organic pollutants. However, it is also an opportunistic pathogen for human and many reports demonstrated that D. acidovorans has strong resistance to aminoglycosides and polymyxins.OBJECTIVE:
The aim of this work was to reveal the antibiotic resistance genes and pathogenic genes in a novel conditional pathogenic strain-D. acidovorans B804, which was isolated from the radiation-polluted soil from Xinjiang Uyghur Autonomous Region, China.METHODS:
The antibiotic resistance test was performed according to the Kirby-Bauer disk diffusion method and evaluated by the standards of the Clinical and Laboratory Standards Institute guidelines. The genome of D. acidovorans B804 was sequenced by a PacBio RS II and Illumina HiSeq 4000 platform in Shanghai Majorbio Biopharm Technology Co., Ltd. (Shanghai, China).RESULTS:
The multidrug resistance phenotypes of D. acidovorans B804 was experimentally confirmed and its genome was sequenced. The total size of D. acidovorans B804 genome was 6,661,314 bp with a GC content of 66.73%. 403 genes associated with antibiotic resistances were predicted. Meanwhile, 89 pathogenic genes were also predicted and 17 of these genes might be capable of causing diseases to human, such as infections and salmonellosis.CONCLUSIONS:
This genomic information can be used as a reference sequence for comparative genomic studies. The results provided more insights regarding the pathogenesis and drug resistance mechanism of D. acidovorans, which will be meaningful for developing more effective therapies toward D. acidovorans-related diseases.Palabras clave
Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Delftia acidovorans
Tipo de estudio:
Guideline
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Biochem Genet
Año:
2023
Tipo del documento:
Article