Profiling Sulfur(VI) Fluorides as Reactive Functionalities for Chemical Biology Tools and Expansion of the Ligandable Proteome.
ACS Chem Biol
; 18(2): 285-295, 2023 02 17.
Article
en En
| MEDLINE
| ID: mdl-36649130
ABSTRACT
Here, we report a comprehensive profiling of sulfur(VI) fluorides (SVI-Fs) as reactive groups for chemical biology applications. SVI-Fs are reactive functionalities that modify lysine, tyrosine, histidine, and serine sidechains. A panel of SVI-Fs were studied with respect to hydrolytic stability and reactivity with nucleophilic amino acid sidechains. The use of SVI-Fs to covalently modify carbonic anhydrase II (CAII) and a range of kinases was then investigated. Finally, the SVI-F panel was used in live cell chemoproteomic workflows, identifying novel protein targets based on the type of SVI-F used. This work highlights how SVI-F reactivity can be used as a tool to expand the liganded proteome.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteoma
/
Fluoruros
Idioma:
En
Revista:
ACS Chem Biol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Reino Unido