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Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques.
Magno da Rocha, Vinícius; Lima, Carla Ormundo Gonçalves Ximenes; Ferreira, Eliane de Oliveira; de Farias, Gabriel Corrêa; Nogueira, Fábio César Sousa; Martha Antunes, Luis Caetano; Cassiano, Keila Mara; Fiorelli, Rossano Kepler Alvim.
Afiliación
  • Magno da Rocha V; Departamento de Cirurgia geral e especializada, Escola de Medicina, Universidade Federal do Estado do Rio de Janeiro (UniRio), Rio de Janeiro- RJ, Brazil.
  • Lima COGX; Departamento de Microbiologia Médica, Institito de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro-RJ, Brazil.
  • Ferreira EO; Departamento de Microbiologia Médica, Institito de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro-RJ, Brazil.
  • de Farias GC; Departamento de Biologia Celular e Molecular, Fundação Oswaldo Cruz, Rio de Janeiro-RJ, Brazil.
  • Nogueira FCS; Instituto de Química, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro-RJ, Brazil.
  • Martha Antunes LC; Centro de Desenvolvimento Tecnológico em Saúde, Fundação Oswaldo Cruz, Rio de Janeiro-RJ, Brazil.
  • Cassiano KM; Departamento de Estatística, Instituto de Matemática, Universidade Federal Fluminense (UFF), Niterói- RJ, Brazil.
  • Fiorelli RKA; Departamento de Cirurgia geral e especializada, Escola de Medicina, Universidade Federal do Estado do Rio de Janeiro (UniRio), Rio de Janeiro- RJ, Brazil.
PLoS One ; 18(2): e0271773, 2023.
Article en En | MEDLINE | ID: mdl-36848344
Lumbar disc degeneration (LDD) and low back pain (LBP) are two conditions that are closely related. Several studies have shown Cutibacterium acnes colonization of degenerated discs, but whether and how these finding correlates with LBP is unknown. A prospective study was planned to identify molecules present in lumbar intervertebral discs (LLIVD) colonized by C. acnes in patients with LDD and LBP and correlate them with their clinical, radiological, and demographic profiles. The clinical manifestations, risk factors, and demographic characteristics of participants undergoing surgical microdiscectomy will be tracked. Samples will be isolated and pathogens found in LLIVD will be characterized phenotypically and genotypically. Whole genome sequencing (WGS) of isolated species will be used to phylotype and detect genes associated with virulence, resistance, and oxidative stress. Multiomic analyses of LLIVD colonized and non-colonized will be carried out to explain not only the pathogen's role in LDD, but also its involvement in the pathophysiology of LBP. This study was approved by the Institutional Review Board (CAAE 50077521.0.0000.5258). All patients who agree to participate in the study will sign an informed consent form. Regardless of the study's findings, the results will be published in a peer-reviewed medical journal. Trials registration number NCT05090553; pre-results.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Dolor de la Región Lumbar / Degeneración del Disco Intervertebral / Disco Intervertebral Tipo de estudio: Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2023 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Dolor de la Región Lumbar / Degeneración del Disco Intervertebral / Disco Intervertebral Tipo de estudio: Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2023 Tipo del documento: Article País de afiliación: Brasil