Interactions of Graphene Oxide and Few-Layer Graphene with the Blood-Brain Barrier.
Nano Lett
; 23(7): 2981-2990, 2023 04 12.
Article
en En
| MEDLINE
| ID: mdl-36917703
ABSTRACT
Thanks to their biocompatibility and high cargo capability, graphene-based materials (GRMs) might represent an ideal brain delivery system. The capability of GRMs to reach the brain has mainly been investigated in vivo and has highlighted some controversy. Herein, we employed two in vitro BBB models of increasing complexity to investigate the bionano interactions with graphene oxide (GO) and few-layer graphene (FLG) a 2D murine Transwell model, followed by a 3D human multicellular assembloid, to mimic the complexity of the in vivo architecture and intercellular crosstalk. We developed specific methodologies to assess the translocation of GO and FLG in a label-free fashion and a platform applicable to any nanomaterial. Overall, our results show good biocompatibility of the two GRMs, which did not impact the integrity and functionality of the barrier. Sufficiently dispersed subpopulations of GO and FLG were actively uptaken by endothelial cells; however, the translocation was identified as a rare event.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Barrera Hematoencefálica
/
Grafito
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nano Lett
Año:
2023
Tipo del documento:
Article
País de afiliación:
Italia