Artificially Evolved Superbinder for Specific Recognition of N6-Methyladenine Base Modification in DNA and RNA.
Anal Chem
; 95(18): 7071-7075, 2023 05 09.
Article
en En
| MEDLINE
| ID: mdl-37098799
ABSTRACT
N6-Methyladenine (m6A/6mA) is a functional epigenetic base modification found in RNA and DNA. By selecting one RNA m6A reader as a template, we created a series of libraries of 3 × 108 RNA m6A reader mutants and developed a yeast surface-display-based evolution approach. Using high-throughput fluorescence-activated cell sorting, we ultimately obtained three evolved 6mA-binding proteins (e6mABPs), which displayed increased affinity for 6mA-containing DNA and reduced affinity for 6mA-free DNA. These e6mABPs are applicable for m6A/6mA enrichment and are potentially applied for modulating cell behavior.
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MEDLINE
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En
Revista:
Anal Chem
Año:
2023
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Article
País de afiliación:
China