Transforming Hairpin-like siRNA-Based Spherical Nucleic Acids into Biocompatible Constructs.
ACS Appl Bio Mater
; 6(9): 3912-3918, 2023 09 18.
Article
en En
| MEDLINE
| ID: mdl-37567247
ABSTRACT
The design and synthesis of hairpin-like small interfering RNA spherical nucleic acids (siRNA-SNAs) based upon biocompatible liposome nanoparticle cores are described. The constructs were characterized by gel electrophoresis, dynamic light scattering, and OliGreen-based oligonucleotide quantification. These siRNA-SNA nanoconstructs enter cells 20-times more efficiently than linear siRNA in as little as 4 h, while exhibiting a 4-fold reduction in cytotoxicity compared with conventional siRNA-SNAs composed of gold nanoparticle cores. Importantly, these siRNA-SNA constructs effectively inhibit angiogenesis in vitro by silencing vascular endothelial growth factor, a key mediator of angiogenesis in a multitude of diseases, in human umbilical vein endothelial cells. This work shows how hairpin architectures can be chemically incorporated into biocompatible SNAs in a way that retains advantageous SNA properties and maximizes gene regulation capabilities.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ácidos Nucleicos
/
Nanopartículas del Metal
Límite:
Humans
Idioma:
En
Revista:
ACS Appl Bio Mater
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos