Automated Cell Counting of Macrophages In Situ.

ABSTRACT

Using the open-source image analysis software CellProfiler to automatically quantify antibody-stained or fluorescently labeled macrophages in situ provides accurate and reproducible cell counts. It is a vastly enhanced alternative method to both manual cell counting and estimation of cell marker expression based on fluorescence intensity. Quantification of tissue-resident macrophages acquired on widefield or confocal microscopes can be batch processed using our pipeline to produce data within minutes.