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Innovative cold atmospheric plasma (iCAP) decreases corneal ulcer formation and bacterial loads and improves anterior chamber health in methicillin resistant Staphylococcus aureus keratitis.
Carr, Mary A; Marquart, Mary E; Sanchez, Miguel; Saleem, Waqar; Wellington, Ocean I; Lovell, K Michael; McCallister, Aaliyah; Lightfoot, Victoria; Ost, Evan B; Wise, Hua Z; Huang, Bo; Sharma, Anjal C.
Afiliación
  • Carr MA; Center for Immunology and Microbial Research and the Department of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS, 39216, USA. Electronic address: mcarr3@umc.edu.
  • Marquart ME; Center for Immunology and Microbial Research and the Department of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS, 39216, USA. Electronic address: mmarquart@umc.edu.
  • Sanchez M; Experimental Therapeutics and Diagnostics Group, Lynntech Inc., 2501 Earl Rudder Fwy South, College Station, TX, 77845, USA. Electronic address: miguel.sanchez@lynntech.com.
  • Saleem W; Experimental Therapeutics and Diagnostics Group, Lynntech Inc., 2501 Earl Rudder Fwy South, College Station, TX, 77845, USA. Electronic address: waqar.saleem@lynntech.com.
  • Wellington OI; Center for Immunology and Microbial Research and the Department of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS, 39216, USA. Electronic address: oceaniris1@gmail.com.
  • Lovell KM; Center for Immunology and Microbial Research and the Department of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS, 39216, USA. Electronic address: klovell@umc.edu.
  • McCallister A; Center for Immunology and Microbial Research and the Department of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS, 39216, USA. Electronic address: amccallister@umc.edu.
  • Lightfoot V; Experimental Therapeutics and Diagnostics Group, Lynntech Inc., 2501 Earl Rudder Fwy South, College Station, TX, 77845, USA. Electronic address: victoria.lightfoot@lynntech.com.
  • Ost EB; Experimental Therapeutics and Diagnostics Group, Lynntech Inc., 2501 Earl Rudder Fwy South, College Station, TX, 77845, USA. Electronic address: evan.ost@lynntech.com.
  • Wise HZ; Experimental Therapeutics and Diagnostics Group, Lynntech Inc., 2501 Earl Rudder Fwy South, College Station, TX, 77845, USA. Electronic address: hua.wise@lynntech.com.
  • Huang B; Department of Ophthalmology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS, 39216, USA. Electronic address: bhuang@umc.edu.
  • Sharma AC; Experimental Therapeutics and Diagnostics Group, Lynntech Inc., 2501 Earl Rudder Fwy South, College Station, TX, 77845, USA. Electronic address: anjal.sharma@lynntech.com.
Exp Eye Res ; 237: 109692, 2023 12.
Article en En | MEDLINE | ID: mdl-37884203
Bacterial keratitis is a vision-threatening infection of the cornea that is typically treated with antibiotics. However, antibiotics sometimes fail to eradicate the infection and do not prevent or repair the damage caused directly by the bacteria or the host immune response to the infection. Our group previously demonstrated that treatment of Pseudomonas aeruginosa keratitis in rabbits with innovative cold atmospheric plasma (iCAP) resulted in reduced edema, ulcer formation, and bacterial load. In this study, we investigated the efficacy of iCAP treatment in methicillin-resistant Staphylococcus aureus (MRSA). New Zealand white rabbits were infected intrastromally with MRSA then treated with iCAP, moxifloxacin, vancomycin, or combination of iCAP with each antibiotic to assess the safety and efficacy of iCAP treatment compared to untreated controls and antibiotics. iCAP treatment significantly reduced bacterial loads and inflammation, improved anterior chamber clarity, and prevented corneal ulceration compared to untreated controls and antibiotic treatment. Safety assessments of grimace test scores and tear production showed that iCAP was not significantly different from either antibiotic treatment in terms of distress or tear production. Combination iCAP/antibiotic treatment did not appear to provide significant added benefit over iCAP alone. Our findings suggest that the addition of iCAP may be a viable tool in reducing damage to the cornea and anterior chamber of the eye following S. aureus keratitis.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Infecciones Bacterianas del Ojo / Úlcera de la Córnea / Staphylococcus aureus Resistente a Meticilina / Queratitis Límite: Animals Idioma: En Revista: Exp Eye Res Año: 2023 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Infecciones Bacterianas del Ojo / Úlcera de la Córnea / Staphylococcus aureus Resistente a Meticilina / Queratitis Límite: Animals Idioma: En Revista: Exp Eye Res Año: 2023 Tipo del documento: Article