Your browser doesn't support javascript.
loading
Quantitative Detection of Biological Nanovesicles in Drops of Saliva Using Microcantilevers.
Cafolla, Clodomiro; Philpott-Robson, James; Elbourne, Aaron; Voïtchovsky, Kislon.
Afiliación
  • Cafolla C; Department of Physics, Durham University, Durham DH1 3LE, U.K.
  • Philpott-Robson J; Department of Physics, Durham University, Durham DH1 3LE, U.K.
  • Elbourne A; School of Science, STEM College, RMIT University, Melbourne, VIC 3001, Australia.
  • Voïtchovsky K; Department of Physics, Durham University, Durham DH1 3LE, U.K.
ACS Appl Mater Interfaces ; 16(1): 44-53, 2024 Jan 10.
Article en En | MEDLINE | ID: mdl-38157306
ABSTRACT
Extracellular nanovesicles (EVs) are lipid-based vesicles secreted by cells and are present in all bodily fluids. They play a central role in communication between distant cells and have been proposed as potential indicators for the early detection of a wide range of diseases, including different types of cancer. However, reliable quantification of a specific subpopulation of EVs remains challenging. The process is typically lengthy and costly and requires purification of relatively large quantities of biopsy samples. Here, we show that microcantilevers operated with sufficiently small vibration amplitudes can successfully quantify a specific subpopulation of EVs directly from a drop (0.1 mL) of unprocessed saliva in less than 20 min. Being a complex fluid, saliva is highly non-Newtonian, normally precluding mechanical sensing. With a combination of standard rheology and microrheology, we demonstrate that the non-Newtonian properties are scale-dependent, enabling microcantilever measurements with a sensitivity identical to that in pure water when operating at the nanoscale. We also address the problem of unwanted sensor biofouling by using a zwitterionic coating, allowing efficient quantification of EVs at concentrations down to 0.1 µg/mL, based on immunorecognition of the EVs' surface proteins. We benchmark the technique on model EVs and illustrate its potential by quantifying populations of natural EVs commonly present in human saliva. The method effectively bypasses the difficulty of targeted detection in non-Newtonian fluids and could be used for various applications, from the detection of EVs and viruses in bodily fluids to the detection of molecular clusters or nanoparticles in other complex fluids.
Asunto(s)
Palabras clave

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Nanopartículas / Vesículas Extracelulares / Neoplasias Límite: Humans Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Nanopartículas / Vesículas Extracelulares / Neoplasias Límite: Humans Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article